PRODUCTION OF BIOSURFACTANTS (RHAMNOLIPIDS) BY Pseudomonas aeruginosa ISOLATED FROM COLOMBIAN SLUDGES
نویسنده
چکیده
he biosurfactant production by strains of Pseudomonas aeruginosa isolated from Colombian hydrocarbon contaminated sludges has been determined. The methodology included the isolation of microorganisms, standardization of batch culture conditions for good surfactant production and characterization of the produced rhamnolipid. Several carbon sources were evaluated with regard to the growth and production curves. The stability of the rhamnolipid was also determined under variable conditions of pH, temperature and salt concentration. The strain Pseudomonas aeruginosa BS 3 showed biosurfactant production capabilities of rhamnolipid resulting in concentrations up to 2 g·dm-3 with surface tensions of 30 32 mN·m-1 in batch cultures with commercial nutrients.
منابع مشابه
Improved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant
A Pseudomonas aeruginosa mutant derived by random mutagenesis with N-methyl-N¢-nitro-N-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on Siegmund-Wagner (SW) plates. The mutant designated P. aeruginosa PTCC1637 produces rhamnolipids at concentrations 10 time more than parent strain. NMR analysis and surface tension measurement showed that the biosurfactan...
متن کاملImproved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant
A Pseudomonas aeruginosa mutant derived by random mutagenesis with N-methyl-N-nitro-Nnitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on SiegmundWagner (SW) plates. The mutant designated P. aeruginosa PTCC1637 produces rhamnolipids at concentrations 10 time more than parent strain. NMR analysis and surface tension measurement showed that the biosurfactants...
متن کاملBiofilm as a production platform for heterologous production of rhamnolipids by the non-pathogenic strain Pseudomonas putida KT2440
BACKGROUND Although a transition toward sustainable production of chemicals is needed, the physiochemical properties of certain biochemicals such as biosurfactants make them challenging to produce in conventional bioreactor systems. Alternative production platforms such as surface-attached biofilm populations could potentially overcome these challenges. Rhamnolipids are a group of biosurfactant...
متن کاملCharacterization by electrospray ionization and tandem mass spectrometry of rhamnolipids produced by two Pseudomonas aeruginosa strains isolated from Brazilian crude oil.
In this work, biosurfactants produced by two Pseudomonas aeruginosa strains isolated from Brazilian crude oils were identified by proton nuclear magnetic resonance ((1)H NMR) and further characterized by mass spectrometry (MS) coupled with electrospray ionization (ESI) and tandem mass spectrometry (MS/MS) analysis in positive mode and their surface activities evaluated. Mono-rhamnolipids and di...
متن کاملRhamnolipid biosurfactants—past, present, and future scenario of global market
RHAMNOLIPIDS—BRIEF OUTLINE Biosurfactants, widely known as surfaceactive agents of biological origin, have carved a niche for themselves in the market due to their unique environmentfriendly properties. They have come a long way since first biosurfactant “surfactin” was purified and characterized by Arima et al. (1968). Biosurfactants have been researched thoroughly and satisfactorily since the...
متن کامل